| 质粒名称: | pHybrid-mApple-Citrobacter-(-2 and 3) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002101 |
| 产品用途: | Expression of Citrobacter |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Citrobacter |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳动物表达优化 |
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pHybrid-mApple-Citrobacter-(-2 and 3)载体质粒基本信息
pHybrid-mApple-Citrobacter-(-2 and 3)质粒图谱载体图谱和pHybrid-mApple-Citrobacter-(-2 and 3)载体序列质粒序列多克隆位点信息
载体图谱.png)
pHybrid-mApple-Citrobacter-(-2 and 3)质粒载体简介
pHybrid-mApple-Citrobacter-(-2 and 3)质粒序列载体序列
LOCUS pHybrid-mApple-Citrobacter-(-2 and 3) 5363 bp DNA circular SYN 16-OCT-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHybrid-mApple-Citrobacter-(-2+3)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5363)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported Apr 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5363
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 138..517
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 518..721
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 754..872
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
primer_bind 889..909
/label=PCI-F
CDS 913..930
/codon_start=1
/label=6x-HisTag
/translation="HHHHHH"
primer_bind complement(949..966)
/label=FR-R1
regulatory 961..966
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 967..1419
/codon_start=1
/label=N-mApple
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
primer_bind 1401..1443
/label=FR--2+3-F
primer_bind 1401..1441
/label=FR--1+3-F
primer_bind 1401..1438
/label=FR-2+3-F
primer_bind 1401..1436
/label=FR-0+3-F
primer_bind 1401..1436
/label=FR-3+3-F
primer_bind complement(1402..1419)
/label=mApple-N-R1
primer_bind 1403..1437
/label=FR-E11-F1
CDS 1420..1860
/label=E11-Citrobacter
primer_bind complement(1834..1890)
/label=FR-1+-2-R
primer_bind complement(1835..1890)
/label=FR-1+-1-R
primer_bind complement(1838..1890)
/label=FR-1+0-R
primer_bind complement(1843..1889)
/label=FR-1+1-R
primer_bind complement(1845..1880)
/label=FR-E11-R1
primer_bind complement(1847..1891)
/label=FR-1+2
CDS 1861..1869
/codon_start=1
/label=Linker2
/translation="SAP"
CDS 1870..2127
/codon_start=1
/label=C-mApple
/translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
primer_bind 1870..1890
/label=C-mApple-F2
primer_bind complement(2108..2127)
/label=0821_FRCaMPi_R
primer_bind complement(2111..2150)
/label=c-mapple-PCI-R
primer_bind 2113..2127
/label=0821_KA_FRCaMPi_F
primer_bind 2131..2158
/label=rrnB-F
primer_bind complement(2131..2152)
/label=mNeo-C-R1
primer_bind 2132..2151
/label=FR-F1
terminator 2143..2237
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
polyA_signal 2261..2382
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2397..2641
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2492..2627
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2629..2670
/label=vector-F1
primer_bind complement(2629..2647)
/label=vector-R1
regulatory 2642..2647
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 2652..2699
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 2779..3234
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2974..3018)
/label=R2
primer_bind 2998..3018
/label=F2
promoter 3566..3670
/gene="bla"
/label=AmpR promoter
CDS 3671..4531
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4641..4684)
/label=R3
primer_bind 4665..4686
/label=F
rep_origin 4702..5290
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tcgtttagtg aaccgtcaga tcactagaag cttcaccaca attcagcaaa ttgtgaacat
781 catcacgttc atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt
841 cgcgaattca ggcgcttttt agactggtcg taacccgttt ttggctaaca ggaggaatta
901 accatgggga gccaccatca ccatcaccat gctagcctcg agaattcacg cgtggtacca
961 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
1021 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
1081 gagggccgcc cctacgaggc cttccagacc gctaggctga aggtgaccaa gggtggcccc
1141 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacatt
1201 aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg
1261 gagcgcgtga tgagcttcga ggacggcggc attattcacg ttaatcagga ctcctccctg
1321 caggacggcg tattcatcta caaggtgaag ctgctcggca ccaacttccc ccccgacggc
1381 cccgtaatgc agaagaagac catgggctgg gaggcttccc tgtttaactt tgttaaagac
1441 gcgggggaaa aactgtggga cgcggttacg gggaaccacg acacggcgga cctggcaaaa
1501 aaagttcagg aacatctgaa taaaaccggt attccggatg cagataaagt tgatgttcag
1561 attaatgaag gtaaagcaac cgttagcggt gatggtctga gccaggaagc aaaagaaaaa
1621 attctgattg caattggtaa tattagcggt attgcaagcg ttgatgatca ggttaaaacc
1681 agcgcaccgg caaacgaaag ccagttttat accgttaaaa gcggtgatac cctgagcgca
1741 attgcaaaac aggtttatgg taatgcaaat ctgtataatc gtatttttga agcaaataaa
1801 ccgatgctga aaagcccgga taaaatttat ccgggtcagg ttctgcgtat tccggaacag
1861 tctgccccgg tttccgagtg gatgtacccc gaggacggcg ccctgaagag cgagatcagg
1921 aaggggctga ggctgaagga cggcggccac tacgccgccg aggtcaagac cacctacaag
1981 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcgagtt ggacatcttg
2041 tcccacaacg aggactacac catcgtggaa cagtgcgaac gcgccgtggg ccgccacccc
2101 accggtggca cggacgcgct gtacaagtag tctagagtcg acccaggcat caaataaaac
2161 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
2221 tcctgagtag gacaaatgcg gccgcttcga gcagacatga taagatacat tgatgagttt
2281 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
2341 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgggtg
2401 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2461 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
2521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
2581 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
2641 agccacctga ccggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttcg
2701 gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
2761 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
2821 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
2881 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
2941 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
3001 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3061 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
3121 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
3181 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
3241 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
3301 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
3361 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
3421 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
3481 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
3541 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
3601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
3661 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
3721 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
3781 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
3841 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
3901 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
3961 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
4021 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
4081 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
4141 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
4201 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
4261 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
4321 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
4381 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
4441 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
4501 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
4561 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
4621 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
4681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
4741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
4801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
4861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
4921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
4981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
5041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
5101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
5161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
5221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
5281 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
5341 tgctcacatg gctcgacaga tct
//
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